Crystal structure of isocitrate lyase from Magnaporthe grisea
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چکیده
منابع مشابه
Isocitrate lyase from Phycomyces blakesleeanus
Isocitrate lyase was purified from Phycomyces blakesleeanus N.R.R.L. 1555(-). The native enzyme has an Mr of 240000. The enzyme appeared to be a tetramer with apparently identical subunits of Mr 62000. The enzyme requires Mg2+ for activity, and the data suggest that the Mg2+-isocitrate complex is the true substrate and that Mg2+ ions act as a nonessential activator. The kinetic mechanism of the...
متن کاملNoteMolecular Structure of rDNA RepeatUnitin Magnaporthe grisea JSeeA
Magnaporthe grisea is an ascomycete that is the causal agent of rice blast disease. The disease is one of the most destructive diseases of rice for the significant loss of the crop. The major strategies for defense against the disease are drugs and resistant cultivars, However, the fungus has been known to be highly variable with respects to drug resistance and host cultivar specificity, and th...
متن کاملExploring the Biology of Magnaporthe grisea
■ Abstract The blast fungus Magnaporthe grisea causes a serious disease on a wide variety of grasses including rice, wheat, and barley. Rice blast is the most serious disease of cultivated rice and therefore poses a threat to the world’s most important food security crop. Here, I review recent progress toward understanding the molecular biology of plant infection by M. grisea, which involves de...
متن کاملStructure and function of 2,3-dimethylmalate lyase, a PEP mutase/isocitrate lyase superfamily member.
The Aspergillus niger genome contains four genes that encode proteins exhibiting greater than 30% amino acid sequence identity to the confirmed oxaloacetate acetyl hydrolase (OAH), an enzyme that belongs to the phosphoenolpyruvate mutase/isocitrate lyase superfamily. Previous studies have shown that a mutant A. niger strain lacking the OAH gene does not produce oxalate. To identify the function...
متن کاملDisappearance of isocitrate lyase enzyme from cells of Chlorella pyrenoidosa.
1. When acetate-adapted cells of Chlorella are suspended in nitrogen-free medium and supplied with glucose, isocitrate lyase activity disappears from the cells at a rate of about 9%/h. This loss of activity is shown to be accompanied by loss of isocitrate lyase protein. 2. When isocitrate lyase activity is assayed in intact cells after freezing and thawing, the rate of loss of activity after ad...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations and Advances
سال: 2014
ISSN: 2053-2733
DOI: 10.1107/s2053273314083326